![]() The second equation we're gonna cover is using the M plus one peak, the height of it to go back and look at how maney, Carbon City originally having a molecule. So, um, the equations that we're gonna cover today are one calculating the height of an M plus one, so sometimes you're going to be asked to estimate How tall would this M plus one be based on what the structure is and we're never You're not expected to get it perfectly right, because this is an approximation, but we can get pretty darn close. No, it's also something you need to build to make do some calculations based on because it's such a consistent ratio that we can actually build equations to solve problems with this. Well, guys, it turns out that this isn't something that you just have to mentally. But you sold this tiny little peek at 17 and I told you, Don't worry about it yet. Remember in our intro video how we were looking at methane and methane Haddon mtz of 16. It's such only 1% but distinctive M plus one peak that's proportional in size. So what this is going to do is it's gonna add a small, very small, because it's so. ![]() ![]() Remember that carbon is usually 12 but it turns out that 1.1% of all the carbon in the universe in fact, 1% of all the carbon that's making up your body has an extra neutron in it. And the M plus one peak is probably the most famous situation where this happens, and it's due to the isotope of carbon 13. Plus has happened because of isotopes, okay. So we've talked a lot about and minus one and minus a lot of numbers, but we haven't talked yet about the pluses. I need to take that into consideration, right? So we're gonna talk about a few different types of peaks that results purely on the because there's isotopes present. So I need to know if there's Adams that out there, that is the same Adam with different weights. Now, why would that be important for mass spectrometry? Because guys we're analyzing Wait. So what that means is that isotopes are gonna have different ways from each other, depending on which Isis hope it iss. But it has a different number of neutrons. So before we go any further, let's just all remember that an isotope would be an atom that has the same number of protons, meaning it's the same element. In this video, we're going to cover the role of isotopes in mass spectrometry.
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